qPCR NEWS - Nov 2010 - 1st Announcement qPCR 2011 Event

Editor www.Gene-Quantification.info via methods%40net.bio.net (by editor from gene-quantification.info)
Thu Nov 25 08:49:51 EST 2010

qPCR NEWS - Nov 2010 - 1st Announcement qPCR 2011 Event

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

    * 1st announcement qPCR 2011 Event in Freising-Weihenstephan,
Germany  =3D> http://www.qPCR2011.net
    * various updates - MIQE media & press review
    * qPCR Application Workshops  -  and more ... ... ...


qPCR 2011 Event - 5th international qPCR Symposium  &  Industrial
Exhibition  &  Application Workshop
28th March - 1st April 2011
in  Freising-Weihenstephan, Technical University of Munich,
Weihenstephan,  Germany


On behalf of the Organisation Committee and the Scientific Board it is
a great pleasure to invite you to the 5th International qPCR Symposium
& Industrial Exhibition & Application Workshop to be held at the
Center of Life Science in Freising Weihenstephan, Technische
Universit=E4t M=FCnchen (Germany). The great international interest in the
previous meetings (qPCR 2004, qPCR 2005, qPCR 2005 in Leipzig, qPCR
2007, qPCR 2009, and qPCR 2010 in Vienna] with up to 600 participants
coming from 56 countries, and over 40 international companies in the
qPCR Industrial Exhibition led us continue the qPCR symposium in
spring 2011.

We have set the date for the  qPCR 2011 Event  to 28th March - 1st
April 2011. The event location is the central lecture hall complex and
the foyer at TUM (Technical University of Munich) in Freising
Weihenstephan, Germany. The TUM and the Biotech region around Munich
is part of the largest Biotech cluster in Europe, located close to the
Munich airport in the heart of Bavaria.

The focus of the qPCR 2011 Event will be on:   Molecular
Diagnostics:   from single-cells  to  Next Generation Sequencing

Leading academic researchers and industrial contributors in the field
will participate in the symposium, which will be an arena for fruitful
discussions between researchers of different backgrounds. The
Symposium Talks, Poster Sessions, Industrial Exhibition and associated
qPCR Application Workshops offer an overview of the present knowledge
and future developments in qPCR and gene expression measurement
technology and its wide applications.

The symposium will focus on 60-70 lectures and more than 100 posters
will be presented by internationally recognised experts in their
field. The emphasis will be on unbiased, didactic information
exchange. Internationally reknown speakers will be participating in a
lively and exciting programme enabling the valuable exchange of
information in the qPCR field. One third of the talks will be
presented by selected invited speakers, one third will be selected
from the submitted abstracts and one third will be presented by qPCR
related company R&D representatives. All scientific contributions will
be published in the qPCR 2011 Symposium Proceedings.
Download 1st qPCR 2011 symposium announcement  =3D>

The qPCR 2011 Event contains several parts:

* qPCR Symposium   March 28th - 30th
  =3D> talk and poster sessions
  =3D> confirmed academic and industrial invited speakers

* A parallel qPCR Industrial Exhibition   March 28th - 30th

* Followed by three qPCR application Workshops   March 31st - April
  powered by the TATAA Biocenter & MultiD Sweden
  - Basic real-time qPCR Application Workshop  (2-days)
  - qPCR data analysis: Biostatistics & Expression Profiling  (2-days)
  - MIQE guidelines (1 day)   &   Practical primer design  (1-day)

Symposium Sessions:
- Molecular diagnostics in single-cells
- High throughput analysis in qPCR & Next Generation Sequencing
- MIQE and QM strategies in qPCR
- small RNAs qPCR - microRNA and siRNA Applications
- Digital PCR  &  Nano-fluidics
- qPCR BioStatistics & BioInformatics

The scientific organization is managed by international well-known
scientists in the field of real-time PCR:
- Stephen Bustin,  Prof. of Molecular Science QM, School of Medicine,
London, UK
- Mikael Kubista,  Prof. of Biotechnology, TATAA Biocenter, Sweden
- Jo Vandesompele,  Prof. at the Center of Medical Genetics,
University of Ghent, Belgium
- Heinrich H.D. Meyer,  Prof. of Physiology, Technical University of
Munich, Germany
- Michael W. Pfaffl,  Reader in Physiology, TUM, Weihenstephan,
  scientific coordinator of the Symposium and the Application
Workshops   qPCR2011 from wzw.tum.de

Event organization:  Dr. Martina Reiter, BioEPS GmbH, Freising,
Germany    Martina.Reiter from BioEPS.com

We are looking forward to meeting you in March 2011 at the Symposium
in Freising-Weihenstephan

    Michael W. Pfaffl           Martina Reiter
    Symposium Chair             BioEPS GmbH

Please register here =3D>  http://registration.qPCR2011.net

Please submit your abstract here =3D>  http://submission.qPCR2011.net


MIQE press & media review

Life Technologies=92 TaqMan Assays QPCR Guarantee Program Sets New
Industry Standard for Customer Service and Support
by Sam Raha, Vice President and General Manager of Genomic Assays  19
Nov 2010
Today we are launching Life Technologies=92 TaqMan Assays QPCR Guarantee
Program, which is designed to provide customers with unparalleled
peace of mind by offering to replace any of our more than 7 million
pre-designed TaqMan=AE assays that don=92t meet their expectations (see
The program helps take some of the risk out of their research by
guaranteeing the quality, performance, content, and results (hence the
acronym =93QPCR=94), across Life Technologies=92 line of pre-designed
TaqMan=AE Assays. If for any reason a pre-designed assay does not
perform to the level of our customers=92 satisfaction, Life Technologies
will first help troubleshoot the issue and, if unsuccessful, we will
replace the assay or credit their account.
This is Life Technologies=92 way of formalizing our commitment to our
customers by standing behind the industry=92s best-performing and most-
consistent pre-designed qPCR assays.
TaqMan=AE pre-designed assays are used in laboratories around the world
among clinical, pharmaceutical, agricultural and academic
researchers.  They serve as powerful tools to rapidly and precisely
measure genomic and proteomic changes in studies that are applied
toward disease research, drug discovery, and agricultural development.
Certain restrictions apply. For details and complete terms and
conditions regarding the TaqMan=AE Assays QPCR Guarantee, please visit

Gene Expression Assay Performance Guaranteed With the TaqMan Assays
QPCR Guarantee Program
Real-time or quantitative PCR (qPCR) is one of the most powerful and
sensitive techniques available for gene expression analysis. It is
used for a broad range of applications, including quantification of
gene expression, measuring RNA interference, biomarker discovery,
pathogen detection, and drug target validation. When studying gene
expression with qPCR, scientists usually investigate changes=97increases
or decreases=97in the quantity of particular gene products or a set of
gene products. Investigations typically evaluate gene response to
biological conditions such as disease states, exposure to pathogens or
chemical compounds, the organ or tissue location, or cell cycle or
differentiation status.

Making the most of MIQE - BMC Molecular Biology 2010
The Editorial Board of BMC Molecular Biology endorse a new set of
essential MIQE-light guidelines for the reporting of quantitative PCR
data: "MIQE precis", and provide guidance for the suitability of pure
reference gene papers to the journal.

MIQE precis: Practical implementation of minimum standard guidelines
for fluorescence-based quantitative real-time PCR experiments
Stephen A Bustin, Jean-Francois Beaulieu, Jim Huggett, Rolf Jaggi,
Frederick SB Kibenge, Pal A Olsvik, Louis C Penning email and Stefan
BMC Molecular Biology 2010 - Published:     21 September 2010
The conclusions of thousands of peer-reviewed publications rely on
data obtained using fluorescence-based quantitative real-time PCR
technology. However, the inadequate reporting of experimental detail,
combined with the frequent use of flawed protocols is leading to the
publication of papers that may not be technically appropriate. We take
the view that this problem requires the delineation of a more
transparent and comprehensive reporting policy from scientific
journals. This editorial aims to provide practical guidance for the
incorporation of absolute minimum standards encompassing the key assay
parameters for accurate design, documentation and reporting of qPCR
experiments (MIQE precis) and guidance on the publication of pure
'reference gene' articles.

MIQE precis: with reference to reference genes - BioMed Central Blog -
Tuesday Sep 21, 2010
Genes that maintain constant expression under a variety of
circumstances are known as =91reference genes=92. They are vital for
researchers who need to quantify gene expression changes in other
genes and need a =91reference point=92 against which to do so. BMC
Molecular Biology, has to date published around 200 reference gene-
related papers from researchers working in such diverse models as
peaches, sharks, barnacles and glioblastoma to name but a few.

... ... ... and much more on  =3D>  MIQE-press.gene-quantification.info


BioEPS GmbH - qPCR Application Workshops

Life Science is still a growing sector and new methods and
technologies are continously developed. Therefore permanent training
and education becomes so important.

With our specific course program we are offering a range of high-
quality course modules, in cooperation with different companies to
give a general and independent overview of existing qPCR technologies
and systems. Our course issues are based on skilled know-how from own
research studies and publications.

Our aim is to point out a critical way of thinking to increase the
quality and outcome of experimental data.

All courses are held regularly in Freising-Weihenstephan, Germany, in
German and English language.
Further customized workshops and specialized trainings will be held as
well across Europe and world-wide.
Workshops are powered by BioEPS GmbH, located at the campus of the
Technical University of Munich, in Freising-Weihenstephan, very close
to the Munich Airport (MUC). For more information and registration,
please see our web page =3D> http://workshops.gene-quantification.info/

Course Occasions 2010:

3-day qPCR Basic Module
2-day BioStatistics & Expression Profiling Module
3-day single-cell qPCR
2-day microRNA qPCR
1-day HRM
2-das qPCR-R data analysis   NEW !

Course dates 2011:

17 -19 Janurary 2011  (E)   3-day qPCR Basic Module (Mon. - Wed.)
21 - 23 February 2011  (E)   3-day qPCR Basic Module (Mon. - Wed.)
28 February - 2 March 2011  (E)   3-day single-cell qPCR Module (Mon.
- Wed.)

Download course brochure =3D> http://www.gene-quantification.de/bioeps-cour=

Register here =3D> http://site.bioeps.com/index.php?option=3Dcom_seminar&It=

Access to our workshops =3D> http://www.gene-quantification.de/bioeps-acces=


Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages


If this newsletter is not displayed correctly by your email client,
please use following link:

The qPCR NEWS and the Gene Quantification Pages are educational sites
with the only purpose of facilitating access to qPCR related
information on the internet.  The qPCR NEWS and the Gene
Quantification Pages are edited by Michael W. Pfaffl.  Copyright =A9
2005 - 2010  All rights reserved.  Any unauthorized use, reproduction,
or transfer of this message or its contents, in any medium, is
strictly prohibited. Disclaimer & Copyrights are displayed on the
homepage  www.gene-quantification.com
To subscribe or change your e-mail address in qPCR NEWS, and if you
would like to receive future issues FREE of charge, please send an e-
mail with the subject SUBSCRIBE to  mailto:newsletter from gene-

More information about the Methods mailing list