does anybody have a recipe for flow cytometry sheath fluid?

Mary via methods%40net.bio.net (by giemsacyto from gmail.com)
Tue Nov 30 05:23:48 EST 2010


On Nov 26, 9:36 am, "Haviland, David L" <David.L.Havil... from uth.tmc.edu>
wrote:
> As I run a flow core, I also use just plain old 0.9% saline as well, but... I know of other flow cytometry centers that use DI as sheath.   I B.S. you not... For the short duration of laminar flow, the central stream of sample fluid (whatever your cells are in) is centered within the DI sheath.    No one really cares what happens to the cells once past the interrogation point, let alone the waste line.   DI also tends to keep the machines cleaner and a lot less crystallization around the SIP tube.
>
> Pointed question:  Have I made the switch?   Not yet.   I have about 60L of 10X sheath to use up, but a colleague across the way uses DI, and I'm going to switch after that 60L is gone.
>
> David Haviland, PhD
> UTHSC- Houston Stem Cell Flow Center.
>
> ________________________________________
> From: methods-boun... from oat.bio.indiana.edu [methods-boun... from oat.bio.indiana.edu] On Behalf Of DK [d... from no.email.thankstospam.net]
> Sent: Wednesday, November 24, 2010 19:11
> To: meth... from magpie.bio.indiana.edu
> Subject: Re: does anybody have a recipe for flow cytometry sheath fluid?
>
> In article <de1ec71b-61bc-496f-979e-43a8b78b9... from u9g2000pra.googlegroups.com>, Mary <giemsac... from gmail.com> wrote:
>
> >Hi All,
> >> the liquid stream (sheath fluid), which carries and aligns the cells
> >> so that they pass single file through the light beam for sensing in
> >> flow cytometers does anybody knowhow to make a DIY sheath fluid? I
> >> thought maybe using 0.9% saline solution would work...amy thoughts?
> >> regards
>
> Pretty much. A simple PBS is what most everyone uses. E.g., here
> is 1X composition:http://www.biosure.com/rpages/solutions.htm
>
> Make it 10X and filter through 0.2 micron and it won't go bad even
> when non-sterile and without preservatives.
>
> DK
> _______________________________________________
> Methods mailing list
> Meth... from net.bio.nethttp://www.bio.net/biomail/listinfo/methods

David why do I need to filter sterilise the PBS?


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