SDM in pBAD24 for complementation

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Fri Sep 17 08:03:03 EST 2010


Hi Arpita,

depends on what you need to do with the plasmid. Which feature has
pBAD24 which features the other one? Which are needed for your
experiment? If your construct is designed well, you should be able to
cut it out easily and insert it in the other vector, if really
necessary.

/Wo

On Sep 17, 12:04 pm, apu <bt.arp... from gmail.com> wrote:
> Dear all,
>
> I have done SDM using Quick Change method by Pfu turbo to insert 72
> bases in a gene cloned in pBAD24 for complementation. Now I got to
> know that I should have done this SDM in some other plasmid and then
> sub clone in pBAD24. Therefore now I have to again sub clone my
> mutated genes to new pBAD24.
>
> My question is that is it necessary? Is any published work there where
> people have used direct SDM pBAD24 plasmid for complementation?
>
> Thanks,
> Arpita



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