(by pjie2 from cam.ac.uk)
Sat Sep 25 20:54:58 EST 2010
On 26/09/2010 02:13, DK wrote:
> In article<8g7dvnFjcgU1 from mid.individual.net>, Peter Ellis<pjie2 from cam.ac.uk> wrote:
>> A consideration I hadn't explained is that I'm distinguishing between an
>> X gene and an autosomal relative, so a peptide antibody was necessary to
>> direct it to regions of greatest divergence between the two. As for the
>> ease or otherwise of expressing proteins in E. coli, that's kind of the
>> problem here!
> Well, probably not for all of the protein's fragments?
True, true, though at some point the distinction between "protein
fragment" and "peptide" starts to become a little blurred! I'll bear
this in mind when making antibodies in future. The other project I'm
working on involves distinguishing between three protein families with
>=90% amino acid identity.
Good luck doing that without peptide ABs... but that's a whole different
story. Not a new story if I'm honest, if you do a search on this
newsgroup, it was a couple of years ago that I first started noticing
I'd ended up with a particularly recalcitrant set of target genes.
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