energy value for UV crosslinking

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Fri Apr 1 06:05:37 EST 2011


Dear Sudheer,

any suggestions from the company your lab has bought it from?

In case of doubt, just run a time series of background and (if
possible) sample (eg several spots on the membrane, cover them with a
piece of cardboard and expose them for various times by stepwise
removal of the shield. Then you will obtain optimal parameters. This
will take in account possible ageing of the UV light source.

Wo

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