Cucl2 interacts with 5x sds sample buffer??

sudheer babu via (by sudheer.pbm07 from
Wed Apr 20 05:19:27 EST 2011

Dear all
I added Copper to my protein sample. After the reaction, when I add 5x
sample buffer for do the PAGE, sample doesnt turns to blue colour but I
noticed some precipitation with yellow color. Then I heated it up and Load
the sample on the SDS-page but I could nt see anything on the gel after
destain it.

Could any one please me in this

Thank you in advance.

Sudheer Babu.S
ITC student
Institute of Biochemistry
Biological Research Center

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