Competitive Assay

Juan Li via methods%40net.bio.net (by jli from custombiologics.com)
Tue Apr 26 08:53:36 EST 2011


Hello everyone,

I would like to run a competitive assay between unlabeled ligand and
FITC-labeled ligand. How should the 2 compounds be incubated, together in a
single incubation step or one after another? What's the advantage or
disadvantage of them?

Any suggestions?

Thanks!
Juan



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