DSP nuclear crosslinking

Naveen Vankadari via methods%40net.bio.net (by naveenis4u from gmail.com)
Fri Apr 29 14:51:41 EST 2011


Hi,

I prefer to use KCl instead of NaCl in your buffer system, it really
important play role. In addition, it is better to ensure that your target
protein is localised into nucleus before you perform nuclear extraction. I
hope this helps you, Good luck.

-- 
Yours Sincerely,
*Naveen Vankadari* ( 那維)
Institute of Molecular Biology,
ACADEMIA SINICA,
Taipei, Taiwan


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