false positive colones in colony hybridization

[Reza Ramzannejad Ghadi]

Dears,

Does anybody have some ideas about false positive colones in colony hybridization? 
Any experinece regarding colony hybridization would be appreciable.
I did it for about 1200 colones but I had just some false positive colones and not any real one.
Best
Reza

 
Reza Ramezannejad Ghadi 
Dep. of Cellular and Developmental Biology of Plants 
University of  Bielefeld, D -33615,  Bielefeld, Germany

Tel: +49-521-1065584 /  +49-176-84090823
http://www.uni-bielefeld.de/biologie/Zellbiologie/index.html
http://www.phycologists.persianblog.ir