Amplify Software Program and Mac OS X Lion
(by R.Jayakumar from roswellpark.org)
Tue Aug 9 09:49:22 EST 2011
There are large number of programs to check primers on the web. There were great algorithms around the web long before programs like amplify. I would use Netprimer to check out primers. But also search the site www.expasy.ch for more free online tools.
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of Jones, Michael D
Sent: Tuesday, August 09, 2011 7:51 AM
To: methods from net.bio.net
Subject: Amplify Software Program and Mac OS X Lion
Not sure if this is the right place, but here goes.
I use Bill Engels "Amplify" program (works only on a Mac OS X, up to 10.6.8). It is imho a great little program for checking out primers for use in PCR.
However, it is written for a PowerPC Mac, and will not work with the new Mac Lion OS.
Anybody know if it will be written to work with Lion, or know of an alternative?
Amplify is not a program designed to 'produce' primer sequences for you, but in silico mimics a PCR reaction. It checks if there are alternative binding sites for your primers within a target region, not just perfect matches, but regions with mismatches, etc.
I never order an oligonucleotide for PCR until I'm happy that it produces a single priming site with Amplify.
MRC Clinical Sciences Centre
Imperial College London
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