formamide for DNA

Cathal Garvey via methods%40net.bio.net (by cathalgarvey from gmail.com)
Fri Aug 19 12:02:18 EST 2011


I have seen glycerol used at 2.5% or 5% for this purpose, though I'm not
sure if that was just "voodoo".
I believe you can use alcohols, also?
Finally, increasing alkalinity will probably affect Tm I'd say, though this
would obviously impact enzyme function for PCR or restriction.

On 19 August 2011 17:13, Ma,Jin <jinma from mdanderson.org> wrote:

> Dear Sir./Madam,
> Do you know any other non or lower toxic chemicals which can attfect the Tm
> of DNA?
> Thanks.
> Jin Ma
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
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>



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