Anti His-tag antibodies in indirect immunofluorescence?
olivier.leger from merckgroup.com
(by olivier.leger from merckgroup.com)
Tue Dec 6 11:45:09 EST 2011
You should try the following reagent which works well in our hands at 1
polyHistidine antibody (monoclonal Mouse IgG1 clone # AD1.1.10) R&D
systems cat# MAB050
From: dk from no.email.thankstospam.net (DK)
To: methods from magpie.bio.indiana.edu
Date: 06-12-11 17:08
Subject: Anti His-tag antibodies in indirect immunofluorescence?
Sent by: methods-bounces from oat.bio.indiana.edu
I need to find out localization pattern for a 8His-tagged protein.
(This came up unexpectedly; had I planned it, there would be
a good tag, 3HA/Flag/Myc). My understanding is that all existing
anti-polyHis monoclonals are rather low affinity. Yet, now there
seems to be a bewildering variety of various anti His-tag Ab
commercially available. Anyone has hands on experience with
using them in indirect immunofluorescence?
Any comments, sources, typical dilutions that don't stain
everything , etc? They are pricey so I can't try everything and
find out what works best.
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