Hi...is there any best and easy way to concentrate the protein
without modifying its original structure
(by usivakumartnau from gmail.com)
Wed Dec 7 01:59:35 EST 2011
try using centricon with appropriate MW cut off or ethanol ppt or AS ppt
can work for you to concentrate the protein.
On Tue, Dec 6, 2011 at 10:27 PM, sudheer sangeetham <sudheer.pbm07 from gmail.com
> Hi Guys
> My recombinant protein is going to inclusion bodies so I am denaturing it
> Gn-HCl and renaturing with GSH and GSSG then finally purified the protein.
> After purification I am getting lower concentration of the protein of
> course it is in larger volume of the buffer. Now I wanted to concentrate
> the protein....Could anyone please tell me, the best and easy way to
> concentrate the protein with out modifying its original structure.
> Thank you in advance.
> Sudheer Babu.S
> Ph.D student
> Institute of Biochemistry
> Biological Research Center
> Methods mailing list
> Methods from net.bio.net
Sivakumar Uthandi PhD
Associate Professor (Ag. Microbiology)
Department of Agrl. Microbiology
Tamil Nadu Agricultural University, Coimbatore 641 003, INDIA
usivakumartnau from gmail.com
914226611294 (O), 914222442055 (R), 918903611294 (Mobile), 914226611394(Fx)
More information about the Methods