quantifying PCR bands

Pragnya Das via methods%40net.bio.net (by drpragnyadas from gmail.com)
Thu Feb 17 14:22:40 EST 2011


I want to quantitate the bands from a Western Blot (X-ray film) and a PCR
gel (Thermal paper). How can I do that. I have heard people using Photoshop
to qunatitate. Is it scientifically accepted. If so, how do I proceed after
scanning the films? I know a lot of labs use ImageJ software.

Your suggestions will be greatly appreciated

PD


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