quantifying PCR bands

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Fri Feb 18 05:39:10 EST 2011


Don't know about photoshop. ImageJ (http://rsbweb.nih.gov/ij/) can do
the task very well. Despite it's free software, it is Java based and
runs in practically every computer environment. As a plus, the mailing
list and support is very active.
To convert the data that you will get into meaningful ones, you'll
probably need a standard curve within the actual picture. A problem
you might run into is the very low dynamic range of xray film and
thermal printouts. If you have the possibility, use fluorescence
scanners (e.g. Licor Odyssey) and cameras instead.

HTH

Wo


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