quantifying PCR bands

Irit Rappley via methods%40net.bio.net (by irappley from scripps.edu)
Fri Feb 18 10:31:26 EST 2011


In addition to establishing the linear range of your film using a 
standard curve as Wo suggests, be sure that your bands are not 
saturated. Everything should be within the grayscale, no true black (for 
black bands on white background) or white (for white bands on black 
background).

Irit



On 2/18/2011 2:39 AM, WS wrote:
> Don't know about photoshop. ImageJ (http://rsbweb.nih.gov/ij/) can do
> the task very well. Despite it's free software, it is Java based and
> runs in practically every computer environment. As a plus, the mailing
> list and support is very active.
> To convert the data that you will get into meaningful ones, you'll
> probably need a standard curve within the actual picture. A problem
> you might run into is the very low dynamic range of xray film and
> thermal printouts. If you have the possibility, use fluorescence
> scanners (e.g. Licor Odyssey) and cameras instead.
>
> HTH
>
> Wo
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