Oil Red O staining problem

Chris McDermott-Roe via methods%40net.bio.net (by cjmcdermottroe from googlemail.com)
Mon Jan 17 12:58:04 EST 2011


Hey everyone,

I'm staining some liver sections with Oil Red O according to a simple
protocol I found at IHC world (
http://www.ihcworld.com/_protocols/special_stains/oil_red_o_ellis.htm) and
noticed that once I apply the mounting media (Histomount HS103 -
http://www.nationaldiagnostics.com/product_info.php/cPath/25_28_35/products_id/188),
my stain diffuses out of the tissue.  The tissue is definitely stained prior
to mounting as it has the characteristic red globule-like lipid dropswhen
viewed at x20/x40.  Is this due to the fact that Histomount is non-aqueous?

any help much appreciated

Chris


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