schistome snail host tissue isolation, storage & DNA extraction
(by sandysu57 from bellsouth.net)
Sat Jan 22 09:20:38 EST 2011
I've done many extractions of snail tissue, but never for a study of parasites.
I don't know what tissue the schistosomes live in, but I've always dissected a
little bit of foot tissue out of the snails and preserved in 95% ethanol at room
temp. I remove the operculum, cut some muscle away and use that. You can also
place the whole animal in 95% ethanol and dissect later -- I actually prefer to
do this because the various types of tissue appear much more different from each
other and are easier to identify after they have been preserved. Some snails
can close the operculum so tightly that the alcohol cannot get inside the shell,
though; so it's always a good idea to crack the shell with a hammer before you
drop the whole thing into ethanol. Otherwise the tissue may start to
Molecular Biology Lab Researcher
Florida State University
From: Dieudonne Agudah <agudahdieu23 from yahoo.com>
To: methods from magpie.bio.indiana.edu
Sent: Wed, January 19, 2011 5:01:28 AM
Subject: schistome snail host tissue isolation, storage & DNA extraction
I had been facing some difficulties with how to isolate tissue of schistosome
host snail from the shell, and its preservation for optimum dna extraction
anytime in future.
May you kindly assist me as to how to go about this.
Count greatly on your support.
Noguchi Memorial Institute for Medical Research
University of Ghana
Legon – Accra
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