proteoglycan removal from extracted RNA

Sia Ghazvini via methods%40net.bio.net (by sghazvini from yahoo.com)
Thu Jul 28 21:04:09 EST 2011


Hi Sara,

You might want to look at a product that might work- RapidClean from advansta.  

Sia

Sent from my iPhone

On Jul 28, 2011, at 12:59 PM, "Sara Edge" <sedge4 from hboi.fau.edu> wrote:

> Does anyone know of a method for removing proteoglycan contaminants from
> total RNA extracts? I work with marine invertebrates (corals) and
> certain species are difficult due to RNA that cannot be successfully
> manipulated downstream (i.e. labeling, RT, etc.). Other research have
> suggested that endogenous proteoglycans interfere with enzymes that bind
> RNA. I use the TRIzol method of extraction and the Qiagen MinElute kit
> for purifications. I am getting good quality RNA based on nanodrop
> spectrophotometry (260:280 @ 2.0) and Agilent Bioanalyzer analysis
> (clean gels, RIN @ 8-9). My 260:230's could be better and tend to be all
> over the place, but have gotten up to 1.9. I would appreciate any
> suggestions.
> 
> 
> Thank you.
> 
> 
> 
> Sara Edge, Ph.D.
> 
> Marine Ecosystem Health
> 
> Harbor Branch Oceanographic Institute at FAU
> 
> 5600 US-1 North, Fort Pierce, FL 34946
> 
> 772-242-2261
> 
> sedge4 from hboi.fau.edu
> 
> 
> 
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