Nickel affinity purification (mnr mnr) (mnr mnr)

Mark Nance via methods%40net.bio.net (by mrnance from umich.edu)
Tue Jun 7 12:55:58 EST 2011


Hi Matt,

Decreasing the NaCl will allow you to do an ion-exchange column if  
your Ni-NTA eluate is not pure enough.  The Qiagen NiNTA handbook  
(QIAexpressionist; packed with information) recommends 300 mM NaCl to  
prevent non-specific binding while still allowing a sufficiently tight  
interaction between the his-tag and the column.  Plus, depending on  
your downstream application, 500 mM NaCl may interfere with whatever  
you're looking for.

On Jun 5, 2011, at 1:04 PM, methods-request from oat.bio.indiana.edu wrote:

> Re: Nickel affinity purification (mnr mnr) (mnr mnr)



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