Biotin-streptavidin - will the bond survive SDS? Urea-Thiourea-CHAPS?

WS via (by novalidaddress from
Wed Jun 22 03:30:39 EST 2011

I agree with DK, there is plentiful of Sulfo-NHS-activated dyes and
respective affinity-labels.

Yoram's initial propasal to use activated Biotin triggered on
question. As biotin is supposed to enter cells by some (transporter -
is there any? which?) mechanism, wouldn't one predominately label this
mechanism instead of "all" surface proteins?

I'm just curiuous...


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