Biotin-streptavidin - will the bond survive SDS?
(by novalidaddress from nurfuerspam.de)
Wed Jun 22 03:30:39 EST 2011
I agree with DK, there is plentiful of Sulfo-NHS-activated dyes and
Yoram's initial propasal to use activated Biotin triggered on
question. As biotin is supposed to enter cells by some (transporter -
is there any? which?) mechanism, wouldn't one predominately label this
mechanism instead of "all" surface proteins?
I'm just curiuous...
More information about the Methods