Transfection

denis guenette via methods%40net.bio.net (by dkguenette from gmail.com)
Fri Jun 24 08:13:12 EST 2011


There is a new transfection reagent called Gylcofect. It is a poly-
glycoamidoamine (PGAA) polymer. Techulon=92s non-lipid reagent
encapsulates nucleic acid and delivers as polyplex with caveolae and
clathrin mediated uptake (1). These polymers are designed to hydrolyse
upon endocytosis, thereby increasing plasmid delivery with lower
cytotoxicity.

Improving Transgene Expression and Cell Viability Using a
Biodegradable Polymeric Delivery Platform
http://www.techulon.com/UserFiles/file/Techulon_TechNote.pdf

Novel Twist on Primary Cell Transfection, Biodegradable Polymer-Based
DNA Carrier Designed to Facilitate Process in Mammalian Cells
http://www.techulon.com/content.aspx?ContentID=3D1362

See for yourself how we can deliver your genes of interest.


Techulon
denis.guenette from techulon.com
www.techulon.com
Office number: 540-443-9254

Ref.

1. McLendon P.M. et al., Poly(glycoamidoamine) Vehicles Promote pDNA
Uptake
through Multiple Routes and Efficient Gene Expression via Caveolae-
Mediated Endocytosis. Molecular Pharmaceutics 2010 Vol. 7(3), 738-750.


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