Phelan, Paul J.
(by Paul.Phelan from tufts.edu)
Tue Mar 1 12:58:09 EST 2011
This may have been asked before (maybe by me), but does anyone have any good advice on how to disrupt GST self-association? I often have trouble with preps. of GST-fusion proteins (when I need the intact fusion protein) being contaminated with GST that is very difficult to get rid of. I think this may be a result of "free" GST associating tightly with the GST-fusion. Has anyone else had this problem, and solved it?
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