Why bl-21 use as protein exression strain?

Pow Joshi via methods%40net.bio.net (by pow.joshi from gmail.com)
Tue Mar 15 10:54:04 EST 2011


you can find the genotype on any of the commercial websites from where you
buy the strain:

for BL21 (DE3) it is:



F–, *omp*T, *hsd*SB(rB-, mB-), *dcm*, *gal*, λ(DE3)
*For *BL21(DE3) pLysS*F–, *omp*T, *hsd*SB(rB-, mB-), *dcm*, *gal,* λ(DE3),
pLysS (Cmr)

Pow


On 14 March 2011 18:54, sudheer babu <sudheer.pbm07 from gmail.com> wrote:

> Hi people
> Can anyone please tell me why Bl-21 use as Protein expression strain?
>
> --
> Sudheer Babu.S
> ITC student
> Institute of Biochemistry
> Biological Research Center
> Szeged,Hungary.
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