Can anyone please tell about slope function

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Mon Mar 21 07:19:52 EST 2011


Dear Sudheer,

the slope is the "m" in your equation. In real life, colorimetric
protein measurement standard curves sometimes get a bit far from
linear, so in times of computerized curve fits by eg excel,
sigma{plot, stat} or openoffice.org, you might be better off with a
2nd order fit (y=ax2 + bx + c); simply plot the OD readings on the x-
axis and the protein standard concentrations on the y-axis, then
you'll get directly the formula to calculate actual concentrations
from the OD values when performing a regression analysis.

By having a look at the R value you may compare various regression
methods (like linear vs 2nd order) and determine which one fits the
situation better.

BTW, the linear approximation is just an approximation which fits most
needs and is easily done when you have just a ruler and a sheet of
paper (when one wouldn't want to apply for mainframe computing time
just for a protein determination :). The chemistry that converts
protein into color is not really linear in respect of dose/response.

Have fun!

Wo


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