Protocol for GSH affinity matrix

José Ramalho via methods%40net.bio.net (by jose.ramalho from fcm.unl.pt)
Sat Mar 26 10:59:45 EST 2011


Dear Dr Ashendel

I am trying to purify recombinant proteins in large amounts, please  
could you send your protocol for synthesis of GSH affinity matrix,  
activation, coupling, and blocking. Thank you.



Best regards

José


__________________________________________
José Ramalho
Investigador Principal
Laboratório de Biologia Celular e Molecular
Faculdade de Ciências Médicas  - Universidade Nova de Lisboa
Campo Mártires da Pátria, 130
1169-056 LISBOA
PORTUGAL

Tel: +351 21 880 30 33
Fax: +351 21 880 30 10



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