Lysis buffer for mouse liver

WS via (by novalidaddress from
Wed Mar 30 04:48:50 EST 2011

On 29 Mrz., 22:45, d... from (DK) wrote:
> In article <mailman.580.1301430500.20886.meth... from>, ijpi... from wrote:
> >Dear all,
Hi DK,

would Laundry Detergent also work as replacement for proteinase K in
mouse tail biopsy genotyping? Or does it also contain DNAse?

Just curious. Probably saving some bucks on proteinase K while risking
a transgenic mouse project wouldn't make much sense...


> >Can anyone suggest a goof recipe for a lysis buffer that efficiently lyses
> >mouse liver and suitable for use in quantification of 55Fe by scintilation
> >counting?
> What's the purpose of the lysis? If you just need to get as much stuff
> as possible into solution then I'd suggest using 1-5% laundry detergent.
> That will really do it, particularly with heating. And very compatible with
> most scintillation cocktails.
> If, however, you need to keep somehting intact/functional then it
> really depends upon what that something is.
> DK

More information about the Methods mailing list