Guanidinium chloride + PBS/N3 -> jelly?
(by novalidaddress from nurfuerspam.de)
Thu Mar 31 10:04:15 EST 2011
Just prepared some 5M GuHCl in PBS/N3 (without Ca and Mg). It didn't
dissolve completely (got a turbid solution) and I decided to filter it
(tissue culture style bottle top filter). I got the expected clear
solution, but in the filter remained a layer of a colourless gelatin
like looking material.
Is this an impurity of the GuHCl did it react with PBS and formed
something like Guanidine phosphate?
More information about the Methods