Guanidinium chloride + PBS/N3 -> jelly?

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Thu Mar 31 10:04:15 EST 2011


Just prepared some 5M GuHCl in PBS/N3 (without Ca and Mg). It didn't
dissolve completely (got a turbid solution) and I decided to filter it
(tissue culture style bottle top filter). I got the expected clear
solution, but in the filter remained a layer of a colourless gelatin
like looking material.

Is this an impurity of the GuHCl did it react with PBS and formed
something like Guanidine phosphate?

Any clues?

Wo


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