qPCR NEWS - November 2011 - focus on mirtrons

Editor www.Gene-Quantification.info via methods%40net.bio.net (by editor from gene-quantification.info)
Thu Nov 24 09:01:14 EST 2011


qPCR NEWS - November 2011 - focus on mirtrons
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Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and RT-qPCR), which are compiled and summarised on the
Gene Quantification homepage. The focus of this newsletter issue is:

* Introducing the mirtron - http://mirtron.gene-quantification.info
* MIQE qPCR APP for IOS and Android is available - http://MIQE.gene-quantification.info
* MIQE guidelines - online translation service to CHINESE,  JAPANESE,
KOREAN and RUSSIAN


If this newsletter is not displayed correctly by your email client,
please use following  http://qPCRnews.gene-quantification.info
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Mirtrons were first identified in Drosophila melanogaster and
Caenorhabditis elegans and are a type of microRNA that are located in
the introns of the mRNA encoding genes. Mirtrons are alternative
precursors for microRNA biogenesis. The short hairpin introns use
splicing to bypass DROSHA cleavage, which is otherwise essential for
the generation of canonical animal microRNAs. Mirtrons arise from the
spliced-out introns and are known to function like classical microRNAs
(miRs) and regulate gene expression, by either mRNA destabilisation,
inhibition of the translation or target mRNA cleavage.
=> http://mirtron.gene-quantification.info

selected mirtron papers:

MicroRNA - Introducing the mirtron
Patrick Goymer, Nature Reviews Molecular Cell Biology 2007 (8): 597

And Now Introducing Mammalian Mirtrons
Shih-Peng Chan and Frank J. Slack
Department of Molecular, Cellular and Developmental Biology, Yale
University, USA, Developmental Cell 13 (2007): 605-607

Mammalian mirtron genes
Berezikov E, Chung WJ, Willis J, Cuppen E, Lai EC., Hubrecht
Institute, Utrecht, The Netherlands, Mol Cell. 2007 28(2): 328-336.

Mirtrons - microRNA biogenesis via splicing
Jakub O. Westholm, Eric C. Lai Corresponding Author Contact
Information, E-mail The Corresponding Author
Department of Developmental Biology, Sloan-Kettering Institute, 1275
York Ave, Box 252, NY 10065, USA, Biochimie 93(11): 1897-1904

Intronic microRNA precursors that bypass Drosha processing
J. Graham Ruby, Calvin H. Jan, & David P. Bartel
Nature Letters Vol 448:5 2007

The biogenesis and characterization of mammalian microRNAs of mirtron
origin
Sibley CR, Seow Y, Saayman S, Dijkstra KK, El Andaloussi S, Weinberg
MS, Wood MJ.
Nucleic Acids Res. 2011

Computational and experimental identification of mirtrons in
Drosophila melanogaster and Caenorhabditis elegans
Chung WJ, Agius P, Westholm JO, Chen M, Okamura K, Robine N, Leslie
CS, Lai EC.
Department of Developmental Biology, Sloan-Kettering Institute,
Rockefeller Research Laboratories, New York, USA, Genome Res. 2011
21(2): 286-300

miRNAs in human cancer
Farazi TA, Spitzer JI, Morozov P, Tuschl T.
Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology,
Rockefeller University, New York, NY 10065, USA, J Pathol. 2011
223(2): 102-115

The mirtron pathway generates microRNA-class regulatory RNAs in
Drosophila
Okamura K, Hagen JW, Duan H, Tyler DM, Lai EC.
Memorial Sloan-Kettering Cancer Center, Department of Developmental
Biology, New York, USA, Cell. 2007 130(1): 89-100

MicroRNA biogenesis via splicing and exosome-mediated trimming in
Drosophila
Flynt AS, Greimann JC, Chung WJ, Lima CD, Lai EC.
Developmental Biology Program, Sloan-Kettering Institute, 1275 York
Avenue, Box 252, New York, NY 10065, USA, Mol Cell. 2010 38(6):
900-907

Intronic noncoding RNAs and splicing
Brown JW, Marshall DF, Echeverria M.
Plant Sciences Division, University of Dundee at the Scottish Crop
Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, UK, Trends
Plant Sci. 2008 13(7): 335-342

and much more papers are available here => http://mirtron.gene-quantification.info


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to further scientists and friends who are interested in qPCR !

Best regards,
Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages

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