is denature plasmid undergo transformation???

sudheer sangeetham via methods%40net.bio.net (by sudheer.pbm07 from gmail.com)
Tue Nov 29 11:30:11 EST 2011


Dear Researchmates

I isolated plasmid from zymogen plasmid isolation kit. After pellet down
the bacterial cells, I added 7x lysis buffer(kit reagent) and by mistake I
resuspend the pellet with pipette( suppose I should not resuspend with
pipette). But when I measure plasmid concentration it was 500 ng/mico
liter. I didnt check the plasmid by running agarose gel. One of my labmate
told chormosomal DNA also could have come when I resuspend with pipette.
When I did bacterial transformation with this plasmid I got few colonies.
Could any please tell, did I do any mistake. Shall I do further steps.
-- 
Sudheer Babu.S
Ph.D student
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.


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