RNAlater on whole fish

Edmund Ling via methods%40net.bio.net (by e.ling1 from uq.edu.au)
Wed Oct 12 04:14:19 EST 2011


We are intending to treat an entire fish (about 3.5 cm in length) with RNAlater before dissection to remove specific organs. We would then isolate RNA from these organs for Illumina HiSeq 2000 sequencing.

Could you please provide feedback on the feasibility/utility of treating the whole animal with RNAlater before dissection, as opposed to treating only the organs after dissection, with respect to RNA yield and sequence fidelity.

Thank you.

Kind regards,

Edmund Y S Ling, DPhil | Research Officer
Applied Bioinformatics Group, Australian Centre for Plant Functional Genomics & School of Agriculture & Food Sciences |  The University of Queensland |
t: 61 7 3346 7083 |  f: 61 7 3365 1176 |  e: e.ling1 from uq.edu.au<mailto:e.ling1 from uq.edu.au> | web: www.appliedbioinformatics.com.au<http://www.appliedbioinformatics.com.au/>

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