Hello...Native Page is not polymerising

Jayakumar, R via methods%40net.bio.net (by R.Jayakumar from roswellpark.org)
Fri Oct 21 07:50:43 EST 2011


Sudheer,
   If you need help, you need to provide information on what you did.  You cannot ask people to read up articles for you.  Please provide the protocol briefly.  If a gel does not polymerise, it is usally something to do with the proton donor used and the catalyst, whcih are usually APS and TEMED which helps Bis to crosslink the acrylamide monomers.   The second reason might be badly oxidised or old acrylamide or Bis-acrylamide or their solutions, third reason might be (very unlikely) dirty plates which were not washed properly.  There could be a host of other reasons and variables.  So provide a detailed protocol.  DO NOT attach it.  Bionet does not allow attachments or website addresses.
Jay


________________________________________
From: methods-bounces from oat.bio.indiana.edu [methods-bounces from oat.bio.indiana.edu] On Behalf Of sudheer sangeetham [sudheer.pbm07 from gmail.com]
Sent: Thursday, October 20, 2011 9:14 AM
To: Methods from magpie.bio.indiana.edu
Subject: Hello...Native Page is not polymerising

Hello
Could any please tell me why my Native PAGE is not polymerizing. I have
referred one article, which i mentioned below and did in similar way and
same concentrations but didnt polymerized. Could any one please tell me the
reason.



Thank you very much in advance

Best Regards

--
Sudheer Babu.S
Ph.D student
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.
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