formamide for DNA

chovek69 via methods%40net.bio.net (by ivanoov from gmail.com)
Tue Sep 13 03:20:57 EST 2011


Try 1,5 Pentanediol... it has huge effect on DNA melting (can lower Tm
with more than 10C) and is absolutely non-toxic.


On Aug 19, 8:36 pm, Hiranya Roychowdhury <hroyc... from nmsu.edu> wrote:
> Sodium chloride ... (can be toxic in high enough concentrations, though ;))
>
> Hiranya S. Roychowdhury, Ph.D.
> Associate Professor
> Health & Public Services
> NMSU-Dona Ana Community  College
> 575 527 7725 (office)


>
> ________________________________________
> From: methods-boun... from oat.bio.indiana.edu [methods-boun... from oat.bio.indiana.edu] on behalf of Ma,Jin [ji... from mdanderson.org]
> Sent: Friday, August 19, 2011 10:13 AM
> To: 'meth... from iubio.bio.indiana.edu'
> Subject: formamide for DNA
>
> Dear Sir./Madam,
> Do you know any other non or lower toxic chemicals which can attfect the Tm of DNA?
> Thanks.
> Jin Ma
> _______________________________________________
> Methods mailing list
> Meth... from net.bio.nethttp://www.bio.net/biomail/listinfo/methods



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