Methods Digest, Vol 76, Issue 2

Virash Gupta via methods%40net.bio.net (by virashkgupta from gmail.com)
Thu Sep 15 05:52:28 EST 2011


what is the concentration of formamide and 1,5 Pentanediol to be used
in PCR for lowering melting temperature

On Wed, Sep 14, 2011 at 10:36 PM,  <methods-request from oat.bio.indiana.edu> wrote:
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>   1. Re: formamide for DNA (chovek69)
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> Message: 1
> Date: Tue, 13 Sep 2011 01:20:57 -0700 (PDT)
> From: chovek69 <ivanoov from gmail.com>
> Subject: Re: formamide for DNA
> To: methods from net.bio.net
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>        <4c300175-5e76-49a6-ad39-318203eafb5c from d26g2000yqa.googlegroups.com>
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> Try 1,5 Pentanediol... it has huge effect on DNA melting (can lower Tm
> with more than 10C) and is absolutely non-toxic.
>
>
> On Aug 19, 8:36 pm, Hiranya Roychowdhury <hroyc... from nmsu.edu> wrote:
>> Sodium chloride ... (can be toxic in high enough concentrations, though ;))
>>
>> Hiranya S. Roychowdhury, Ph.D.
>> Associate Professor
>> Health & Public Services
>> NMSU-Dona Ana Community  College
>> 575 527 7725 (office)
>
>
>>
>> ________________________________________
>> From: methods-boun... from oat.bio.indiana.edu [methods-boun... from oat.bio.indiana.edu] on behalf of Ma,Jin [ji... from mdanderson.org]
>> Sent: Friday, August 19, 2011 10:13 AM
>> To: 'meth... from iubio.bio.indiana.edu'
>> Subject: formamide for DNA
>>
>> Dear Sir./Madam,
>> Do you know any other non or lower toxic chemicals which can attfect the Tm of DNA?
>> Thanks.
>> Jin Ma
>> _______________________________________________
>> Methods mailing list
>> Meth... from net.bio.nethttp://www.bio.net/biomail/listinfo/methods
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-- 
Dr V K Gupta
Sr Microbiologist (Mol Biology)
IMBL, Department of Entomology
Pun. Agric. Univ., Ludhiana (Pb)-141004- India
M: 081465-55515



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