Krebs buffer cloudy

Gretha Van Oorschot via methods%40net.bio.net (by gretha.vanoorschot from rmit.edu.au)
Thu Aug 2 01:25:10 EST 2012


Hi I make a lot of Krebs although ours does not have pyruvate.but does have
EDTA and ascorbic acid.  We always make 10x stock and make EDTA CAcl2 and
asc acid separate. and add on the day of use. It always goes cloudy the
moment CaCl2 is added. I would be worried if it didn't. The cloudiness
dissapears when it is bubbled with carbanox.


-- 
 Ms. G. Van Oorschot
Technical Officer
School of Medical Sciences
RMIT University
Bundoora West Campus
Plenty Road,
Bundoora 3083
Phone +61 3 99257323
Fax      +61 3 94678589


More information about the Methods mailing list