Southern Blot hybridization question

Vladimir Gainullin via (by gainullin from
Thu Aug 2 16:41:53 EST 2012

Instead of boiling my probe to denature in labeling solution I accidentally
added it to Express Hyb, then realized my mistake and boiled the whole 30ml
of hybridization buffer + probe for 20 minutes in 95C heating block. Do you
think the SDS and whatever else that might be in Express Hyb is going to
interfere with probe denaturation at 95C?


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