Desperately looking for E. coli with lambda cI857 Sam7
(by blackhole from abuse.plus.com)
Wed Feb 15 10:56:12 EST 2012
Historians believe that in newspost
<26192644.3453.1329291940527.JavaMail.geo-discussion-forums from vbmh19> on
Tue, 14 Feb 2012, Bjorn <bjornjobb from gmail.com> penned the following
>I am looking for an E. coli strain with a lysogenic lambda phage that is practical to induce and purify, such as one with cI857 Sam7.
>If you have one, I would be most grateful if you could send it to me.
>Alternatively, point me to a resource that is free or not too expensive where I could buy one.
Buy some standard full length lambda cI857 Sam7 methylated DNA from NEB
and electroporate into suitable E.coli host. Screen for lysogen i.e.
grows at 28C but not 42C.
Or buy in same DNA but find someone with a lambda packing kit, package,
plate screen etc.
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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