Desperately looking for E. coli with lambda cI857 Sam7

Bjorn via methods%40net.bio.net (by bjornjobb from gmail.com)
Thu Feb 16 02:50:02 EST 2012


On Feb 15, 3:56 pm, Duncan Clark <blackh... from abuse.plus.com> wrote:
> Historians believe that in newspost
> <26192644.3453.1329291940527.JavaMail.geo-discussion-forums from vbmh19> on
> Tue, 14 Feb 2012, Bjorn <bjornj... from gmail.com> penned the following
> literary masterpiece:
>
> >I am looking for an E. coli strain with a lysogenic lambda phage that is practical to induce and purify, such as one with cI857 Sam7.
>
> >If you have one, I would be most grateful if you could send it to me.
> >Alternatively, point me to a resource that is free or not too expensive where I could buy one.
>
> Buy some standard full length lambda cI857 Sam7 methylated DNA from NEB
> and electroporate into suitable E.coli host. Screen for lysogen i.e.
> grows at 28C but not 42C.
>
> Or buy in same DNA but find someone with a lambda packing kit, package,
> plate screen etc.
>
> Duncan
> --
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
>
> Duncan Clark
> GeneSys Ltd.


Thanks a lot! but how do I select for transformants? does it have a
selection marker?
sorry, never worked with lambda...
/bjorn


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