Anti-His Ab reaction with HEK293T cell extract

Phelan, Paul J. via methods%40net.bio.net (by Paul.Phelan from tufts.edu)
Fri Feb 17 14:53:26 EST 2012


I have had problems with an anti-poly-His Ab (GE cat. no. 27-4710-01) picking up something (background) in western blots of HEK 293T cell extracts.  Has anyone else had any experience of this?  Is there a better anti-His antibody that's popular in the western blotting community?

Thanks,

Paul Phelan
Tufts University Dept. of Biochemistry
Boston



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