Anti-His Ab reaction with HEK293T cell extract

[Phelan, Paul J.]

I have had problems with an anti-poly-His Ab (GE cat. no. 27-4710-01) picking up something (background) in western blots of HEK 293T cell extracts.  Has anyone else had any experience of this?  Is there a better anti-His antibody that's popular in the western blotting community?

Thanks,

Paul Phelan
Tufts University Dept. of Biochemistry
Boston