Anti-His Ab reaction with HEK293T cell extract
Phelan, Paul J.
(by Paul.Phelan from tufts.edu)
Fri Feb 17 14:53:26 EST 2012
I have had problems with an anti-poly-His Ab (GE cat. no. 27-4710-01) picking up something (background) in western blots of HEK 293T cell extracts. Has anyone else had any experience of this? Is there a better anti-His antibody that's popular in the western blotting community?
Tufts University Dept. of Biochemistry
More information about the Methods