in vitro delivery of palmitic acid

Wolfgang Schechinger via methods%40net.bio.net (by glybirne from gmail.com)
Mon Feb 20 03:15:16 EST 2012


Hi Daniel,

possibly you have more luck with vesicles. Protocols should be a lot
in the literature. Basically, it's injecting an eg ethanolic solution
of FA and some other stuff (lecithine) through a narrow gauge needle
into buffer and the dialyzing out the EtOH if necessary. Or you dry
the lipid mix down by evaporation, add buffer and prepare the vesicles
by sonication.

HTH

Wo


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