sudheer sangeetham via methods%40net.bio.net (by sudheer.pbm07 from gmail.com)
Tue Jan 3 11:52:41 EST 2012

Hi People

I have few doubts regarding phosphte buffer saline (PBS). Recently I found
one article related to my work, in that they used PBS ( Nacl/iP ) pH-7.4.
Nacl/iP does it mean, it should contain Na2HP04 and NaH2PO4 and Nacl but
not KCl, am I right?

I have prepared the buffer like this

NaH2PO4 ( 0.038M)
Na2HPO4.2H20 ( 0.162M)
NaCl (1.5 M ) but when i check the pH it was showing 6.4, is it correct?
did I do any mistake....

Could any one please tell me did i do any mistake?

Thank you in advance

Sudheer Babu.S
Ph.D student
Institute of Biochemistry
Biological Research Center

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