10X PBS

Joshua Silverstein via methods%40net.bio.net (by silverstein.joshua from gmail.com)
Wed Jan 4 16:07:48 EST 2012


You don't need physical chemistry, just general.

If you take 10X PBS and dilute it with deionized water, it will certainly
change the pH.  For a 10-fold dilution, it should change the pH by
approximately 1 (log scale).



On Wed, Jan 4, 2012 at 3:38 PM, DK <dk from noemail.thankstospam.net> wrote:

> In article <mailman.75.1325703460.3721.methods from net.bio.net>, "Jayakumar,
> R" <R.Jayakumar from roswellpark.org> wrote:
> >Ideally the pH should not change much between 10X and 1X if deionised
> water was
> > used for dilution.  Presnce of NaCl has nothing to do with pH changes
> since it
> > contributes neither hydroxyl nor hydrogen ions
>
> You need to refresh your physical chemistry.
> (Hint: cations compete with protons for binding to anions)
>
> DK
>
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