10X PBS

Jayakumar, R via methods%40net.bio.net (by R.Jayakumar from roswellpark.org)
Thu Jan 5 12:07:58 EST 2012


Dk,
  Finally, something we agree on :-))
Jay


-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of DK
Sent: Thursday, January 05, 2012 11:25 AM
To: methods from magpie.bio.indiana.edu
Subject: Re: 10X PBS

In article <mailman.77.1325727966.3721.methods from net.bio.net>, Joshua Silverstein <silverstein.joshua from gmail.com> wrote:
>You don't need physical chemistry, just general.
>
>If you take 10X PBS and dilute it with deionized water, it will certainly
>change the pH.  For a 10-fold dilution, it should change the pH by
>approximately 1 (log scale).

False. We are talking about buffers here... 

DK



>
>
>On Wed, Jan 4, 2012 at 3:38 PM, DK <dk from noemail.thankstospam.net> wrote:
>
>> In article <mailman.75.1325703460.3721.methods from net.bio.net>, "Jayakumar,
>> R" <R.Jayakumar from roswellpark.org> wrote:
>> >Ideally the pH should not change much between 10X and 1X if deionised
>> water was
>> > used for dilution.  Presnce of NaCl has nothing to do with pH changes
>> since it
>> > contributes neither hydroxyl nor hydrogen ions
>>
>> You need to refresh your physical chemistry.
>> (Hint: cations compete with protons for binding to anions)
>>
>> DK
>>
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