10X PBS

Pow Joshi via methods%40net.bio.net (by pow.joshi from gmail.com)
Mon Jan 9 15:18:01 EST 2012


I like this discussion. A lot of info about old forgotten physical chem :)
Thank you DK, Engelbert, Jayakumar, and others.

On 9 January 2012 03:08, DK <dk from no.email.thankstospam.net> wrote:

> In article <MPG.29740567a625f6369896b0 from News.Individual.DE>, Dr Engelbert
> Buxbaum <engelbert_buxbaum from hotmail.com> wrote:
> >
> >Btw, if one wants to work with intracellular enzymes, PBS should not be
> >used, and replaced with a high K, low Na medium!
>
> Well, if we are that strict then chloride salts should not be used either
> :-)
> Intracellular Cl- concentration is pretty low and most free anions are
> large organics. For "pseudo-intracellular" solution, I've isethionate in
> one system and glutamate in another. Some people like using tartrate.
>
> To be sure, it does make a difference. Halide ions bind proteins a lot.
>
> DK
>
> P.S.  Recently I came across your book, Engelbert. Pretty good!
> Made me envious :-)
>
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