(by R.Jayakumar from roswellpark.org)
Tue Jan 10 12:23:29 EST 2012
My pleasure Pow :-)
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of Pow Joshi
Sent: Monday, January 09, 2012 3:18 PM
Subject: Re: 10X PBS
I like this discussion. A lot of info about old forgotten physical chem :)
Thank you DK, Engelbert, Jayakumar, and others.
On 9 January 2012 03:08, DK <dk from no.email.thankstospam.net> wrote:
> In article <MPG.29740567a625f6369896b0 from News.Individual.DE>, Dr Engelbert
> Buxbaum <engelbert_buxbaum from hotmail.com> wrote:
> >Btw, if one wants to work with intracellular enzymes, PBS should not be
> >used, and replaced with a high K, low Na medium!
> Well, if we are that strict then chloride salts should not be used either
> Intracellular Cl- concentration is pretty low and most free anions are
> large organics. For "pseudo-intracellular" solution, I've isethionate in
> one system and glutamate in another. Some people like using tartrate.
> To be sure, it does make a difference. Halide ions bind proteins a lot.
> P.S. Recently I came across your book, Engelbert. Pretty good!
> Made me envious :-)
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> Methods from net.bio.net
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