"Best" chemiluminiscent Westen detection?
(by sghazvini from yahoo.com)
Wed Jan 18 21:11:21 EST 2012
You can try a free sample of Advansta's WesternBright Quantum through this link:
From: Michael Sullivan <mlsulliv from wisc.edu>
Cc: methods from magpie.bio.indiana.edu
Sent: Wednesday, January 18, 2012 5:07 PM
Subject: Re: "Best" chemiluminiscent Westen detection?
I've not done much comparison, but I've been using Bio-Rad's Immun-Star substrate for years. I use it with PVDF. There's an added enhancer for use with nitrocellulose, but I don't use NC, so I don't now how well that works. I've found the signal very long lived.
Don't know about the price either. I don't do tons of westerns but I've been happy with the product, so cost has not been a big issue. We aren't set up for film anymore. We do detection with our Fotodyne gel doc system that also does luminescent detection.
On Jan 18, 2012, at 4:11 PM, DK wrote:
> It's been over ten years since I last used ELC for Westerns. Back
> then, we used Pierce's "ECL Plus" and a Kodak film of the type I can no
> longer remember.
> It looks like I will be doing a bunch again in the upcoming months.
> Ten years is a long time and I wonder if there were any new developments
> or better/cheaper products available now.
> Super-duper sensitivity is not required. Strong preference is for the
> substrate that has long-living signal (I was never abale to achieve it
> with home-made luminol). Low price would be nice, too. AP or HRP
> - does not matter.
> Oh, and what is a good inexpensive film to buy for ECL these days?
> - Dima
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