GATEWAY CLONING

Philip O'Brien via methods%40net.bio.net (by p.obrien from murdoch.edu.au)
Sat Jun 2 22:43:25 EST 2012


I want to clone PCR products into Gateway vectors.  The vector I¹m using has
attR1 and attR2 sequences for recombinational cloning.  However I need to
attach attL1 and attL2 tags to my primers.  Where can I find the sequences
of attL1 and attL2?

Also if I want to use Invitrogen¹s entry vectors, where can I find the
sequences of these vectors?

Phil
P.Obrien from murdoch.edu.au





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