Methods Digest, Vol 82, Issue 4

Michael Sullivan via (by mlsulliv from
Fri Mar 9 17:24:26 EST 2012

I think this is a needless worry! Water has almost no buffering capacity, so a tiny amount of dissolved CO2 impacts water's pH. This amount of CO2 is very small compared to the amount of ACTUAL buffer (TRIS, phosphate, whatever) you are using, so will generally have only a negligible effect on pH of your buffer solution.

Michael L. Sullivan, PhD
Research Molecular Geneticist
US Dairy Forage Research Center
1925 Linden Drive
Madison, WI 53706
608-890-0046 (Phone)
608-890-0076 (FAX)

On Mar 9, 2012, at 2:35 PM, sudheer sangeetham wrote:

> Hello
> Thank you for the reply. If MQ water pH is 6.0, then I think we should not
> use this MQ for preparation of buffers. If we use it, it will change the
> require buffer pH?
> Cheers
> -- 
> Sudheer Babu.S
> Ph.D student
> Institute of Biochemistry
> Biological Research Center
> Szeged,Hungary.
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> Methods mailing list
> Methods from

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