Ammonium sulfate precipitation from solutions with high sugar content

Pow Joshi via methods%40net.bio.net (by pow.joshi from gmail.com)
Tue May 1 10:58:19 EST 2012


and here's one more, from sugarcane :)
http://onlinelibrary.wiley.com/doi/10.1002/elps.200900779/pdf
on quick view it seems to me that they follow the method that Michael
suggested, however, they also seem to have comparative yields from
different methods ... hope that helps
Pow

On 1 May 2012 11:52, Pow Joshi <pow.joshi from gmail.com> wrote:

> well, here's a plant phloem/Xylem sap protein isolation I found....
> http://www.springerlink.com/content/h8622j4747246137/#section=82175&page=4&locus=23
>
> They use Centricon ultra filtration step to remove the sugars it seems to
> me .... followed by TCA/acetone precipitation ....
>
>
> On 1 May 2012 07:18, WS <novalidaddress from nurfuerspam.de> wrote:
>
>> Hi Michael, I do not need active protein, I need it for a western. I'll
>> give it a try, thanks!
>>
>> Wo
>>
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