Regarding primary antibody binding in western blot

divya teja via methods%40net.bio.net (by divya.medbio from gmail.com)
Wed May 16 08:03:16 EST 2012


hello,

Can I use 2 PVDF  membranes at the same time in single box containing
primary antibody in 10ml 1x PBST while doing western blot?

Will the primary antibody bind properly or gets probed properly, if I use 2
membranes same time in the single box????????

waiting for the reply


regards
divya


More information about the Methods mailing list